Journal: STAR Protocols

Article Title: Generation of hematopoietic lineage cell-specific chimeric mice using retrovirus-transduced fetal liver cells

doi: 10.1016/j.xpro.2022.101526

Figure Lengend Snippet:

Article Snippet: Mouse SCF, research grade , Miltenyi Biotec , 130-101-741.

Techniques: Virus, Recombinant, Marker, Modification, Saline, Transfection, Western Blot, Plasmid Preparation, Purification, Gel Extraction, Retroviral, Sequencing, Software, Flow Cytometry

Journal: BMC Medicine

Article Title: IGF1-mediated mesenchymal-endothelial transition as a potential regulatory target in calcific aortic valve disease

doi: 10.1186/s12916-025-04433-z

Figure Lengend Snippet: IGF1 identified as a key molecule mediating MEndT. A Conduct protein-protein interaction network analysis (PPI) on the overlapping differentially expressed genes between pVECs vs. pVICs and pVICs-OM8d vs. pVICs. B Analyze the FPKM values of SOD2, CCL2, CCN2, IGF1, and COL3A1 in the transcriptome sequencing of pVICs-OM8d vs. pVICs, normalized to the control pVICs group. Values are mean ± SD of 4 biological replicates. Statistical tests used: ANOVA. C , D WB ( C ) and qPCR ( D ) were used to detect the protein expression of IGF1, IGF1R, P-IGF1R, and the mRNA expression levels of IGF1, IGF1R in pVICs cultured for 8 days in GM or OM. D Normalized to pVICs and GAPDH. Values are mean ± SD of 3 independent experiments. Statistical tests used: ANOVA. E , F Statistical analysis of tube formation assays of pVICs with exogenous addition of IGF1 and BMS-536924 during OM induction ( E ), and after knockdown of IGF1 expression. Normalized to GM group ( F ). Normalized to pVICs. Values are mean ± SD. 3 biological replicates, with 3 random measurements within each replicate, n =9. Statistical tests used: ANOVA. G , H Assessed the changes in protein expression ( G ) of CDH5, CD31, α-SMA, PI3K, Akt, P-Akt, and HIF-1α in pVICs after the exogenous addition of recombinant IGF1 and inhibitor BMS-536924 in GM and OM, and changes in mRNA expression levels ( H ) of CDH5, CD31, α-SMA. H Normalized to pVICs and GAPDH. Values are mean ± SD of 3 independent experiments. Statistical tests used: ANOVA. I , J Detect the protein ( J ) and mRNA ( I ) expression of CDH5, CD31, α-SMA in pVICs after knockdown of IGF1 expression during OM induction. I Normalized to pVICs and GAPDH. Values are mean ± SD of 3 independent experiments. Statistical tests used: ANOVA. K , L Tube formation assays in pVICs with exogenous addition of IGF1 and BMS-536924 during OM induction, and after knockdown of IGF1 expression, scale bars: 200 µm

Article Snippet: Each group received intraperitoneal injections every 2 days with either saline (100 μl), recombinant mouse IGF1 protein (1 μM, 100 μl; MCE, HY-P7070), or BMS-536924 (1 mg; MCE, HY-10262).

Techniques: Sequencing, Control, Expressing, Cell Culture, Knockdown, Recombinant

Journal: BMC Medicine

Article Title: IGF1-mediated mesenchymal-endothelial transition as a potential regulatory target in calcific aortic valve disease

doi: 10.1186/s12916-025-04433-z

Figure Lengend Snippet: IGF1-mediated MEndT and disease progression in the AVWI mouse mode. A Schematic diagram of the animal experiment procedure. B Echocardiographic evaluation of the sham surgery group and the AVWI mouse groups with intraperitoneal injection of saline, IGF1, or BMS-536924. Parameters assessed included: aortic valve annulus diameter (mm) and transaortic peak velocity (mm/s). C , D Statistical analysis of the aortic valve annulus diameter (mm) and transaortic peak velocity (mm/s) in each group of mice. n =8. Values are mean ± SD. Statistical tests used: ANOVA. E HE staining (scale bars: 200 µm) and multiplex immunofluorescence histology (scale bars: 100 µm, 50 µm, or 20 µm) for CD31, tdTomato, and DAPI of mouse aortic valve paraffin sections. Yellow arrows indicate CD31-positive cells labeled with tdTomato. F , G Statistical analysis of the aortic valve thickness (µm) (HE staining, E ) and tdTomato labeled cells expressing CD31 within the aortic valve region (immunofluorescence staining, G ) in each group of mice. n =8. Values are mean ± SD. Statistical tests used: ANOVA

Article Snippet: Each group received intraperitoneal injections every 2 days with either saline (100 μl), recombinant mouse IGF1 protein (1 μM, 100 μl; MCE, HY-P7070), or BMS-536924 (1 mg; MCE, HY-10262).

Techniques: Biomarker Discovery, Injection, Saline, Staining, Multiplex Assay, Immunofluorescence, Labeling, Expressing

Journal: Cell stem cell

Article Title: The Mitochondrial Transacylase, Tafazzin, Regulates AML Stemness by Modulating Intracellular Levels of Phospholipids

doi: 10.1016/j.stem.2019.02.020

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: mSCF , Miltenyi Biotec , Cat# 130-101-697.

Techniques: Recombinant, Modification, Western Blot, Binding Assay, XF Assay, Electron Microscopy, Staining, High Performance Thin Layer Chromatography, Reverse Transcription, Plasmid Preparation, Apoptosis Assay, DC Protein Assay, SYBR Green Assay, Gene Expression, shRNA, Control, CRISPR, Software